The attenuation phenotype conferred by the M gene of the influenza A/Ann Arbor/6/60 cold-adapted virus (H2N2) on the A/Korea/82 (H3N2) reassortant virus results from a gene constellation effect
Identifieur interne : 001F33 ( Main/Exploration ); précédent : 001F32; suivant : 001F34The attenuation phenotype conferred by the M gene of the influenza A/Ann Arbor/6/60 cold-adapted virus (H2N2) on the A/Korea/82 (H3N2) reassortant virus results from a gene constellation effect
Auteurs : E. Kanta Subbarao [États-Unis] ; Mark Perkins [États-Unis] ; John J. Treanor [États-Unis] ; Brian R. Murphy [États-Unis]Source :
- Virus Research [ 0168-1702 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Adaptation physiologique, Animaux, Basse température, Cricetinae, Données de séquences moléculaires, Femelle, Gènes viraux, Humains, Phénotype, Réplication virale (génétique), Souris, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Séquence nucléotidique, Vaccins antigrippaux (isolement et purification), Vaccins atténués (isolement et purification), Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Virus de la grippe A (physiologie).
- MESH :
- génétique : ADN viral, Réplication virale, Virus de la grippe A.
- immunologie : Virus de la grippe A.
- isolement et purification : Vaccins antigrippaux, Vaccins atténués.
- physiologie : Virus de la grippe A.
- Adaptation physiologique, Animaux, Basse température, Cricetinae, Données de séquences moléculaires, Femelle, Gènes viraux, Humains, Phénotype, Souris, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Séquence nucléotidique.
English descriptors
- KwdEn :
- Adaptation, Physiological, Animals, Base Sequence, Cold Temperature, Cricetinae, DNA, Viral (genetics), Female, Genes, Viral, Humans, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A virus (genetics), Influenza A virus (immunology), Influenza A virus (physiology), Influenza Vaccines (isolation & purification), Mice, Molecular Sequence Data, Phenotype, Vaccines, Attenuated (isolation & purification), Virus Replication (genetics).
- MESH :
- chemical , genetics : DNA, Viral.
- genetics : Influenza A virus, Virus Replication.
- immunology : Influenza A virus.
- chemical , isolation & purification : Influenza Vaccines, Vaccines, Attenuated.
- physiology : Influenza A virus.
- Teeft :
- Adaptation, Physiological, Amino acid, Animals, Attenuated, Attenuation, Attenuation phenotype, Base Sequence, Betts, Cold Temperature, Constellation, Cricetinae, Donor virus, Female, Gene, Gene constellation, Gene constellation effect, Gene segment, Genes, Viral, Genetic stability, Hamster, Humans, Influenza, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza virus, Influenza virus vaccine, Lowest level, Maassab, Mdck cells, Mice, Molecular Sequence Data, Mutation, Nasal turbinates, Nucleotide sequence, Phenotype, Polymerase, Polymerase genes, Present study, Reassortant, Reassortant virus, Reassortant viruses, Replication, Respiratory tract, Sequencing, Snyder, Standard error, Tcid, Turbinate, Vaccine, Viral, Virus, Virus genes, Virus vaccine, Virus vaccines, Wild type virus.
Abstract
Abstract: A single gene reassortant (SGR) virus that derived its M gene from the attenuated influenza A/Ann Arbor/6/60 cold-adapted (CA) donor virus and the remaining genes from the A/Korea/82 (H3N2) wild type (WT) virus (designated A/Korea/82 CA M-SGR) was previously shown to be attenuated in mice, hamsters, ferrets, and humans. The attenuation (ATT) phenotype of this SGR virus could result directly from an altered function of the mutant M gene product of the A/Ann Arbor/6/60 CA virus, which differs from the M gene of the A/Ann Arbor/6/60 WT virus at only one amino acid or, indirectly from a gene constellation effect in which ATT results from an inefficient interaction between the products of the M gene of the A/Ann Arbor/6/60 virus and other genes of the A/Korea/82 virus. Several lines of evidence from the present study are consistent with our interpretation that the ATT phenotype of the A/Korea/82 CA M-SGR results from a gene constellation effect. First, the A/Korea/82 CA M-SGR and an A/Korea/82 SGR containing the A/Ann Arbor/6/60 WT M gene were each restricted in replication in the upper and lower respiratory tract of mice compared with the A/Korea/82 WT virus. Second, an A/Udorn/72 CA M-SGR containing the M gene from the A/Ann Arbor/6/60 CA donor virus in a background of other genes derived from the A/Udorn/72 (H3N2) WT virus was not attenuated in the respiratory tract of mice. These data suggest that the change in the amino acid sequence of the M gene product from the A/Ann Arbor/6/60 WT to CA virus is not responsible for the ATT phenotype of the A/Korea/82 CA M-SGR. In addition, evidence of the genetic instability of the A/Korea/82 CA M-SGR is presented, specifically, an extragenic mutation that results in loss of the ATT phenotype. The implications of these findings for the ATT phenotype of the live attenuated reassortant viruses derived from the A/Ann Arbor/6/60 CA donor virus are discussed.
Url:
DOI: 10.1016/0168-1702(92)90098-T
Affiliations:
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Physiological</term><term>Animals</term><term>Base Sequence</term><term>Cold Temperature</term><term>Cricetinae</term><term>DNA, Viral (genetics)</term><term>Female</term><term>Genes, Viral</term><term>Humans</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Influenza A virus (physiology)</term><term>Influenza Vaccines (isolation & purification)</term><term>Mice</term><term>Molecular Sequence Data</term><term>Phenotype</term><term>Vaccines, Attenuated (isolation & purification)</term><term>Virus Replication (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Cricetinae</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Gènes viraux</term><term>Humains</term><term>Phénotype</term><term>Réplication virale (génétique)</term><term>Souris</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Séquence nucléotidique</term><term>Vaccins antigrippaux (isolement et purification)</term><term>Vaccins atténués (isolement et purification)</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (immunologie)</term><term>Virus de la grippe A (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Réplication virale</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adaptation, Physiological</term><term>Amino acid</term><term>Animals</term><term>Attenuated</term><term>Attenuation</term><term>Attenuation phenotype</term><term>Base Sequence</term><term>Betts</term><term>Cold Temperature</term><term>Constellation</term><term>Cricetinae</term><term>Donor 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error</term><term>Tcid</term><term>Turbinate</term><term>Vaccine</term><term>Viral</term><term>Virus</term><term>Virus genes</term><term>Virus vaccine</term><term>Virus vaccines</term><term>Wild type virus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Cricetinae</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Gènes viraux</term><term>Humains</term><term>Phénotype</term><term>Souris</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Séquence nucléotidique</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A single gene reassortant (SGR) virus that derived its M gene from the attenuated influenza A/Ann Arbor/6/60 cold-adapted (CA) donor virus and the remaining genes from the A/Korea/82 (H3N2) wild type (WT) virus (designated A/Korea/82 CA M-SGR) was previously shown to be attenuated in mice, hamsters, ferrets, and humans. The attenuation (ATT) phenotype of this SGR virus could result directly from an altered function of the mutant M gene product of the A/Ann Arbor/6/60 CA virus, which differs from the M gene of the A/Ann Arbor/6/60 WT virus at only one amino acid or, indirectly from a gene constellation effect in which ATT results from an inefficient interaction between the products of the M gene of the A/Ann Arbor/6/60 virus and other genes of the A/Korea/82 virus. Several lines of evidence from the present study are consistent with our interpretation that the ATT phenotype of the A/Korea/82 CA M-SGR results from a gene constellation effect. First, the A/Korea/82 CA M-SGR and an A/Korea/82 SGR containing the A/Ann Arbor/6/60 WT M gene were each restricted in replication in the upper and lower respiratory tract of mice compared with the A/Korea/82 WT virus. Second, an A/Udorn/72 CA M-SGR containing the M gene from the A/Ann Arbor/6/60 CA donor virus in a background of other genes derived from the A/Udorn/72 (H3N2) WT virus was not attenuated in the respiratory tract of mice. These data suggest that the change in the amino acid sequence of the M gene product from the A/Ann Arbor/6/60 WT to CA virus is not responsible for the ATT phenotype of the A/Korea/82 CA M-SGR. In addition, evidence of the genetic instability of the A/Korea/82 CA M-SGR is presented, specifically, an extragenic mutation that results in loss of the ATT phenotype. The implications of these findings for the ATT phenotype of the live attenuated reassortant viruses derived from the A/Ann Arbor/6/60 CA donor virus are discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li><li>État de New York</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Subbarao, E Kanta" sort="Subbarao, E Kanta" uniqKey="Subbarao E" first="E. Kanta" last="Subbarao">E. Kanta Subbarao</name></region><name sortKey="Murphy, Brian R" sort="Murphy, Brian R" uniqKey="Murphy B" first="Brian R." last="Murphy">Brian R. Murphy</name><name sortKey="Perkins, Mark" sort="Perkins, Mark" uniqKey="Perkins M" first="Mark" last="Perkins">Mark Perkins</name><name sortKey="Treanor, John J" sort="Treanor, John J" uniqKey="Treanor J" first="John J." last="Treanor">John J. Treanor</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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